T cells directed against self-antigens play a pivotal role in autoimmunity, yet their comprehensive characterization in human autoimmune disorders remains challenging. In this chapter, we present methods to isolate and profile autoreactive T cells from human biological samples, specifically peripheral blood, cerebrospinal fluid, and tissue biopsies. The workflow combines in vitro T cell screening, isolation of autoreactive T cells, and single T cell clone generation, followed by in depth analysis of T cell receptor (TCR) repertoire, HLA restriction, cytokine profile, and targeted self-epitope. These protocols aim to facilitate research of human autoimmune diseases by providing powerful tools for systematic analysis of autoreactive T cells in human biological samples.
Identification and Characterization of Human Autoreactive T Cells for Autoimmune Research / Súkeníková, L., Latorre, D.. - 2990:(2026), pp. 1-18. [10.1007/978-1-0716-4997-8_1]
Identification and Characterization of Human Autoreactive T Cells for Autoimmune Research
Latorre, Daniela
2026-01-01
Abstract
T cells directed against self-antigens play a pivotal role in autoimmunity, yet their comprehensive characterization in human autoimmune disorders remains challenging. In this chapter, we present methods to isolate and profile autoreactive T cells from human biological samples, specifically peripheral blood, cerebrospinal fluid, and tissue biopsies. The workflow combines in vitro T cell screening, isolation of autoreactive T cells, and single T cell clone generation, followed by in depth analysis of T cell receptor (TCR) repertoire, HLA restriction, cytokine profile, and targeted self-epitope. These protocols aim to facilitate research of human autoimmune diseases by providing powerful tools for systematic analysis of autoreactive T cells in human biological samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
