Dentine Permeability and Dentine Morphology after Treatment with Sanguinaria Canadensis and Chlorexidine

Toothpastes, mouthwashes, liners, that remove smear layer and increase the functional diameter of dentinal tubules may be responsible for hypersensitivity and pain. The aim of this study to evaluate in vitro the effect on dentin permeability of two mouthwashes proposed for periodontal therapy: Periogard with Sanguinaria Canadensis 0.03% (Colgate, USA) and Periogard with Chlorexidine 0.2% (Colgate, USA). Twenty-four occlusally sectioned crown segments from human extracted third molars were divided into four groups and connected to an in vitro hydraulic apparatus as described by Pashely et al. (1978). All groups were etched with 37% phosphoric acid gel (3M, USA) to remove the smear layer and to calculate the maximum permeability. In two groups, smear layer was recreated using #400 3M sandpaper disks. Finally, the undiluted mouthwashes were applied and brushed on the dentin for 3 minutes with a toothbrush (Colgate, USA). Each sample was then washed with water and dentin permeability was evaluated. Four measurements of permeability (expressed as hydraulic conductance in ml/min per cm H2O) were made for each sample the averaged. ANOVA tests and Paired Student's test were used where appropriate. SEM analysis was made on the same samples.