: Here, we provide detailed protocols for the isolation of mouse Kupffer cells - the liver-resident macrophages - for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).

Isolation of mouse Kupffer cells for phenotypic and functional studies

Andreata, Francesco
Primo
;
De Simone, Giorgia;Fumagalli, Valeria;Kuka, Mirela;Iannacone, Matteo
2021-01-01

Abstract

: Here, we provide detailed protocols for the isolation of mouse Kupffer cells - the liver-resident macrophages - for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).
2021
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Single Cell
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11768/122617
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