Kupffer cells (KCs) are highly abundant, intravascular, liver-resident macrophages known for their scavenger and phagocytic functions. KCs can also present antigens to CD8+ T cells and promote either tolerance or effector differentiation, but the mechanisms underlying these discrepant outcomes are poorly understood. Here, we used a mouse model of hepatitis B virus (HBV) infection, in which HBV-specific naive CD8+ T cells recognizing hepatocellular antigens are driven into a state of immune dysfunction, to identify a subset of KCs (referred to as KC2) that cross-presents hepatocellular antigens upon interleukin-2 (IL-2) administration, thus improving the antiviral function of T cells. Removing MHC-I from all KCs, including KC2, or selectively depleting KC2 impaired the capacity of IL-2 to revert the T cell dysfunction induced by intrahepatic priming. In summary, by sensing IL-2 and cross-presenting hepatocellular antigens, KC2 overcome the tolerogenic potential of the hepatic microenvironment, suggesting new strategies for boosting hepatic T cell immunity.
Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming / De Simone, G.; Andreata, F.; Bleriot, C.; Fumagalli, V.; Laura, C.; Garcia-Manteiga, J. M.; Di Lucia, P.; Gilotto, S.; Ficht, X.; De Ponti, F. F.; Bono, E. B.; Giustini, L.; Ambrosi, G.; Mainetti, M.; Zordan, P.; Benechet, A. P.; Rava, M.; Chakarov, S.; Moalli, F.; Bajenoff, M.; Guidotti, L. G.; Ginhoux, F.; Iannacone, M.. - In: IMMUNITY. - ISSN 1074-7613. - 54:9(2021), pp. 2089-2100.e8. [10.1016/j.immuni.2021.05.005]
Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming
De Simone G.;Fumagalli V.;Laura C.;De Ponti F. F.;Guidotti L. G.;Iannacone M.
2021-01-01
Abstract
Kupffer cells (KCs) are highly abundant, intravascular, liver-resident macrophages known for their scavenger and phagocytic functions. KCs can also present antigens to CD8+ T cells and promote either tolerance or effector differentiation, but the mechanisms underlying these discrepant outcomes are poorly understood. Here, we used a mouse model of hepatitis B virus (HBV) infection, in which HBV-specific naive CD8+ T cells recognizing hepatocellular antigens are driven into a state of immune dysfunction, to identify a subset of KCs (referred to as KC2) that cross-presents hepatocellular antigens upon interleukin-2 (IL-2) administration, thus improving the antiviral function of T cells. Removing MHC-I from all KCs, including KC2, or selectively depleting KC2 impaired the capacity of IL-2 to revert the T cell dysfunction induced by intrahepatic priming. In summary, by sensing IL-2 and cross-presenting hepatocellular antigens, KC2 overcome the tolerogenic potential of the hepatic microenvironment, suggesting new strategies for boosting hepatic T cell immunity.File | Dimensione | Formato | |
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