After allogeneic hematopoietic stem cell transplantation (allo-HSCT), the emergence of circulating Cytomegalovirus(CMV)-specific T cells correlates with protection from CMV reactivation, an important risk factor for non-relapse mortality. However, functional assays measuring CMV-specific cells are time-consuming and often inaccurate at early timepoints. We report results of a prospective single-center non-interventional study which identifies the enumeration of Dextramer-positive CMV-specific lymphocytes as a reliable and early predictor of viral reactivation. We longitudinally monitored 75 consecutive patients for 1 year after allo-HSCT (n=630 samples). The presence of ≥0.5 CMV-specific CD8+ cells//L at day+45 was an independent protective factor from subsequent clinicallyrelevant reactivation in univariate(p.
Cytomegalovirus-specific T cells restricted for shared and donor human leukocyte antigens differentially impact on Cytomegalovirus reactivation risk after allogeneichematopoietic stem cell transplantation / Tassi, Elena; Noviello, Maddalena; De Simone, Pantaleo; Lupo-Stanghellini, Maria T; Doglio, Matteo; Serio, Francesca; Abbati, Danilo; Beretta, Valeria; Valtolina, Veronica; Oliveira, Giacomo; Racca, Sara; Campodonico, Edoardo; Ruggiero, Eliana; Clerici, Daniela; Giglio, Fabio; Lorentino, Francesca; Dvir, Roee; Xue, Elisabetta; Farina, Francesca; Oltolini, Chiara; Manfredi, Francesco; Vago, Luca; Corti, Consuelo; Bernardi, Massimo; Clementi, Massimo; Brix, Liselotte; Ciceri, Fabio; Peccatori, Jacopo; Greco, Raffaella; Bonini, Chiara. - In: HAEMATOLOGICA. - ISSN 1592-8721. - 108:6(2023), pp. 1530-1543. [Epub ahead of print] [10.3324/haematol.2022.280685]
Cytomegalovirus-specific T cells restricted for shared and donor human leukocyte antigens differentially impact on Cytomegalovirus reactivation risk after allogeneichematopoietic stem cell transplantation
De Simone, Pantaleo;Doglio, Matteo;Campodonico, Edoardo;Dvir, Roee;Xue, Elisabetta;Farina, Francesca;Manfredi, Francesco;Vago, Luca;Clementi, Massimo;Ciceri, Fabio;Bonini, Chiara
2023-01-01
Abstract
After allogeneic hematopoietic stem cell transplantation (allo-HSCT), the emergence of circulating Cytomegalovirus(CMV)-specific T cells correlates with protection from CMV reactivation, an important risk factor for non-relapse mortality. However, functional assays measuring CMV-specific cells are time-consuming and often inaccurate at early timepoints. We report results of a prospective single-center non-interventional study which identifies the enumeration of Dextramer-positive CMV-specific lymphocytes as a reliable and early predictor of viral reactivation. We longitudinally monitored 75 consecutive patients for 1 year after allo-HSCT (n=630 samples). The presence of ≥0.5 CMV-specific CD8+ cells//L at day+45 was an independent protective factor from subsequent clinicallyrelevant reactivation in univariate(p.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
