High-mobility group-1 protein (HMG1) was produced in Escherichia coli under the control of the T7 promoter/T7 RNA polymerase system. The protein can be produced and purified with yields similar to those obtained from animal tissues. HMG1 purified from E. coli is homogeneous and capable of selectively binding cruciform DNA, indicating that post-translational processing of vertebrate HMG1 is not necessary for its DNA-binding ability.

Production of functional rat HMG1 protein in Escherichia coli

Bianchi, M E
1991-01-01

Abstract

High-mobility group-1 protein (HMG1) was produced in Escherichia coli under the control of the T7 promoter/T7 RNA polymerase system. The protein can be produced and purified with yields similar to those obtained from animal tissues. HMG1 purified from E. coli is homogeneous and capable of selectively binding cruciform DNA, indicating that post-translational processing of vertebrate HMG1 is not necessary for its DNA-binding ability.
1991
HIGH-MOBILITY GROUP-1 PROTEIN
RECOMBINANT DNA
PROTEIN DNA INTERACTIONS
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11768/136796
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