A hypervirulent strain (CB/05) of canine coronavirus was employed to infect oronasally 11-week-old pups. Peripheral blood monocytes (CD14(+)), T lymphocytes (CD4(+) and CD8(+)) and B lymphocytes (CD21(+)) were studied by flow cytometry within 5 days post-infection (p.i.) and at later time points. Infection with CB/05 resulted in a profound depletion of T cells and a slight loss of B cells in the first week p.i. In particular, while the CD8(+) and the B lymphocytes returned to baseline levels by day 7 p.i., the CD4(+) T cells remained significantly low until day 30 p.i. and recovered completely only at day 60 p.i. Monocytosis was also observed after CB/05 infection with a peak at day 5 p.i. The prolonged depletion of peripheral CD4(+) T cells did not alter the levels of serum IgG or IgM. The impact of CB/05 infection on the immune performance of infected pups is discussed. (C) 2010 Elsevier B.V. All rights reserved.

Prolonged depletion of circulating CD4(+) T lymphocytes and acute monocytosis after pantropic canine coronavirus infection in dogs / Marinaro, M; Mari, V; Bellacicco, Al; Tarsitano, E; Elia, G; Losurdo, M; Rezza, G; Buonavoglia, C; Decaro, N. - In: VIRUS RESEARCH. - ISSN 0168-1702. - 152:1-2(2010), pp. 73-78. [10.1016/j.virusres.2010.06.006]

Prolonged depletion of circulating CD4(+) T lymphocytes and acute monocytosis after pantropic canine coronavirus infection in dogs

Rezza G;
2010-01-01

Abstract

A hypervirulent strain (CB/05) of canine coronavirus was employed to infect oronasally 11-week-old pups. Peripheral blood monocytes (CD14(+)), T lymphocytes (CD4(+) and CD8(+)) and B lymphocytes (CD21(+)) were studied by flow cytometry within 5 days post-infection (p.i.) and at later time points. Infection with CB/05 resulted in a profound depletion of T cells and a slight loss of B cells in the first week p.i. In particular, while the CD8(+) and the B lymphocytes returned to baseline levels by day 7 p.i., the CD4(+) T cells remained significantly low until day 30 p.i. and recovered completely only at day 60 p.i. Monocytosis was also observed after CB/05 infection with a peak at day 5 p.i. The prolonged depletion of peripheral CD4(+) T cells did not alter the levels of serum IgG or IgM. The impact of CB/05 infection on the immune performance of infected pups is discussed. (C) 2010 Elsevier B.V. All rights reserved.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11768/157074
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