The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing / Mancini, F., Barbanti, F., Scaturro, M., Errico, G., Iacobino, A., Bella, A., Riccardo, F., Marsili, G., Stefanelli, P., Pezzotti, P., Rezza, G., Ciervo, A.. - In: EMERGING MICROBES & INFECTIONS. - ISSN 2222-1751. - 9:1(2020), pp. 1393-1396. [10.1080/22221751.2020.1775500]

Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

Rezza G.
Penultimo
;
2020-01-01

Abstract

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
2020
COVID 19
heat treatment
molecular methods
nasopharyngeal samples
rt-Real time PCR
SARS-CoV-2
File in questo prodotto:
File Dimensione Formato  
Laboratory management.pdf

accesso aperto

Tipologia: PDF editoriale (versione pubblicata dall'editore)
Licenza: Creative commons
Dimensione 918.58 kB
Formato Adobe PDF
918.58 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11768/157760
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 38
  • ???jsp.display-item.citation.isi??? 32
social impact