The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing / Mancini, F.; Barbanti, F.; Scaturro, M.; Errico, G.; Iacobino, A.; Bella, A.; Riccardo, F.; Marsili, G.; Stefanelli, P.; Pezzotti, P.; Rezza, G.; Ciervo, A.. - In: EMERGING MICROBES & INFECTIONS. - ISSN 2222-1751. - 9:1(2020), pp. 1393-1396. [10.1080/22221751.2020.1775500]
Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing
Rezza G.Penultimo
;
2020-01-01
Abstract
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.| File | Dimensione | Formato | |
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