Human chromosomal DNA coding for single chain urokinase-type plasminogen activator (scu-PA) has been inserted into a vector and expressed in LB6 cells. The recombinant clone termed 1C9 is stable and produces scu-PA at about 0.8 mg/I/day in a bioreactor. The scu-PA produced is human by amino- and carboxy-terminal analysis and immunoblotting. The recombinant scu-PA is glycosylated and fully activatable by plasmin. © 1989.
Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells / Nolli, M. L.; Sarubbi, E.; Corti, A.; Robbiati, F.; Soflientini, A.; Blasi, F.; Parenti, F.; Cassani, G.. - In: FIBRINOLYSIS. - ISSN 0268-9499. - 3:2(1989), pp. 101-106. [10.1016/0268-9499(89)90038-6]
Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells
Corti A.;Blasi F.;
1989-01-01
Abstract
Human chromosomal DNA coding for single chain urokinase-type plasminogen activator (scu-PA) has been inserted into a vector and expressed in LB6 cells. The recombinant clone termed 1C9 is stable and produces scu-PA at about 0.8 mg/I/day in a bioreactor. The scu-PA produced is human by amino- and carboxy-terminal analysis and immunoblotting. The recombinant scu-PA is glycosylated and fully activatable by plasmin. © 1989.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
