We report new methods of 2 photon polymerization of microlenses with high numerical aperture, large diameter and good optical quality. We characterize the aberrations of these lenses that, coupled to raster scanning optical microscopes, allow two-photon excitation imaging of cells. In-vivo non-linear imaging will be also discussed, opening the possibility to use these micro-lenses in implants for the continuous inspection of biological dynamics in vivo.

Micro-Fabricated Optics for Multiphoton Microscopy / Vazquez, R. M.; Nardini, A.; Conci, C.; Jacchetti, E.; Marini, M.; Panzeri, D.; Sironi, L.; Bouzin, M.; Collini, M.; Inverso, D.; Kariman, B. S.; Kabouraki, E.; Farsari, M.; Osellame, R.; Cerullo, G.; Raimondi, M. T.; Chirico, G.. - 2023-:(2023). ( 23rd International Conference on Transparent Optical Networks, ICTON 2023 rou 2023) [10.1109/ICTON59386.2023.10207360].

Micro-Fabricated Optics for Multiphoton Microscopy

Inverso D.;
2023-01-01

Abstract

We report new methods of 2 photon polymerization of microlenses with high numerical aperture, large diameter and good optical quality. We characterize the aberrations of these lenses that, coupled to raster scanning optical microscopes, allow two-photon excitation imaging of cells. In-vivo non-linear imaging will be also discussed, opening the possibility to use these micro-lenses in implants for the continuous inspection of biological dynamics in vivo.
2023
2-photon polymerization
label free imaging
micro-lenses
non-linear excitation microscopy
optical microscopy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11768/185740
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