: This is an update to the Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), Chapter 3: 12C, by Cossarizza et al. Administration of anti-ARTC2 nanobody(S+16a) prevents cell death during tissue processing. We demonstrate that the phenotype of CD44midTreg is significantly impacted, whereas the eTreg phenotype remains stable following S+16a treatment, outlining specific protocols for population recovery.
Optimising Recovery of Hepatic Regulatory T Cells: A Practical Guide Using ARTC2 Blockade / Abbott, C.; Mouro, V.; Perucchini, C.; Vespari, C.; Iannacone, M.. - In: EUROPEAN JOURNAL OF IMMUNOLOGY. - ISSN 0014-2980. - 55:12(2025). [10.1002/eji.70095]
Optimising Recovery of Hepatic Regulatory T Cells: A Practical Guide Using ARTC2 Blockade
Mouro V.
Secondo
Membro del Collaboration Group
;Perucchini C.
Membro del Collaboration Group
;Vespari C.
Penultimo
Membro del Collaboration Group
;Iannacone M.
Ultimo
Membro del Collaboration Group
2025-01-01
Abstract
: This is an update to the Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), Chapter 3: 12C, by Cossarizza et al. Administration of anti-ARTC2 nanobody(S+16a) prevents cell death during tissue processing. We demonstrate that the phenotype of CD44midTreg is significantly impacted, whereas the eTreg phenotype remains stable following S+16a treatment, outlining specific protocols for population recovery.| File | Dimensione | Formato | |
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Eur J Immunol - 2025 - Abbott - Optimising Recovery of Hepatic Regulatory T Cells A Practical Guide Using ARTC2 Blockade.pdf
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