Objectives: The purpose of this study was to investigate, by morphostructural and immunohistochemical analysis, the process of bone remodeling during the integration of two different extraoral autogenous bone grafts: calvaria and iliac crest. Methods:The donor sites were Calvaria and Iliac Crest, used to fill bone defects in edentulous sites, in posterior maxilla, for future implant therapy. Samples were withdrawn from donor sites at the moment of grafts insertion (T0), while samples from regenerated sites were taken four months (T1) after. Bone specimens were processed to achieve Hematoxilin-Eosin staining to perform light microscope analysis, to investigate the expression of Bone Sialoprotein and the number of apoptotic cells. Results: Both samples at T1 showed strong remodeling phenomena with the presence of new blood vessels, even if in sites regenerated with calvaria grafts a more dynamic aspect could be detected. BSP and BAX were significantly more expressed in samples T1 compared to that observed in samples T0. In any samples from sites treated with calvaria grafts, the investigated molecule BSP showed higher levels of expression than in samples with iliac crest bone grafts. Similarly, the number of apoptotic cells, evaluated by TUNEL analysis, in samples T1 it results higher in calvaria, while in all specimens it seems to be significantly elevated compared to t0. Conclusions: The calvaria graft microscopic aspect at T1 seems to be more organized and regular than that observed in sites regenerated with iliac crest graft at the same experimental time, suggesting a better clinical performance. Those phenomena of bone apposition and osteoblast differentiation are indicated by rising levels of BSP in T1, that it's specifically higher in calvaria bone graft. The results of Tunel analysis, strong indicators of bone remodeling, although giving higher value for the calvaria, showed an equal number of apoptotic cells between calvaria and iliac crest.
MORPHOSTRUCTURAL AND IMMUNOHISTOCHEMICAL EVALUATION OF CALVARIA VS. ILIAC-CREST BONE GRAFTS
VINCI , RAFFAELE;GHERLONE , FELICE ENRICO
2011-01-01
Abstract
Objectives: The purpose of this study was to investigate, by morphostructural and immunohistochemical analysis, the process of bone remodeling during the integration of two different extraoral autogenous bone grafts: calvaria and iliac crest. Methods:The donor sites were Calvaria and Iliac Crest, used to fill bone defects in edentulous sites, in posterior maxilla, for future implant therapy. Samples were withdrawn from donor sites at the moment of grafts insertion (T0), while samples from regenerated sites were taken four months (T1) after. Bone specimens were processed to achieve Hematoxilin-Eosin staining to perform light microscope analysis, to investigate the expression of Bone Sialoprotein and the number of apoptotic cells. Results: Both samples at T1 showed strong remodeling phenomena with the presence of new blood vessels, even if in sites regenerated with calvaria grafts a more dynamic aspect could be detected. BSP and BAX were significantly more expressed in samples T1 compared to that observed in samples T0. In any samples from sites treated with calvaria grafts, the investigated molecule BSP showed higher levels of expression than in samples with iliac crest bone grafts. Similarly, the number of apoptotic cells, evaluated by TUNEL analysis, in samples T1 it results higher in calvaria, while in all specimens it seems to be significantly elevated compared to t0. Conclusions: The calvaria graft microscopic aspect at T1 seems to be more organized and regular than that observed in sites regenerated with iliac crest graft at the same experimental time, suggesting a better clinical performance. Those phenomena of bone apposition and osteoblast differentiation are indicated by rising levels of BSP in T1, that it's specifically higher in calvaria bone graft. The results of Tunel analysis, strong indicators of bone remodeling, although giving higher value for the calvaria, showed an equal number of apoptotic cells between calvaria and iliac crest.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
