A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [Ca2+]i measurements, evidence is provided that these oscillations originate, via the activation of Ca(2+)-induced Ca(2+)-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not. [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.

[Ca2+]i oscillations from internal stores sustain exocytic secretion from the chromaffin cells of the rat.

MALGAROLI , ANTONIO;
1991-01-01

Abstract

A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca2+]i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [Ca2+]i measurements, evidence is provided that these oscillations originate, via the activation of Ca(2+)-induced Ca(2+)-release, from intracellular Ca2+ stores in rapid equilibrium with extracellular Ca2+. By combining [Ca2+]i measurements with a specific plaque secretion assay we demonstrate that oscillating cells exhibit a spontaneous exocytic secretory activity whereas the cells with stable [Ca2+]i do not. [Ca2+]i oscillations appear therefore to account for the high unstimulated catecholamine release, an activity typical of the chromaffin cells of the rat.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11768/3034
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