"\"OBJECTIVE:. To assess local expression and plasma levels of pentraxin 3 (PTX3) in patients with giant cell arteritis (GCA).. METHODS:. Plasma and serum samples were obtained from 75 patients with GCA (20 of whom had experienced optic nerve ischemia in the previous 3 weeks and 24 of whom had experienced symptom onset in the previous 6 months and had no history of optic nerve ischemia) and 63 controls (35 age-matched healthy subjects, 15 patients with rheumatoid arthritis, and 13 patients with chronic stable angina). In 9 patients in whom GCA was recently diagnosed, circulating levels of interleukin-1β (IL-1β), IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12p70, CCL2\\\/monocyte chemotactic protein 1, CCL3\\\/macrophage inflammatory protein 1α (MIP-1α), CCL4\\\/MIP-1β, CCL11\\\/eotaxin, CXCL9\\\/monokine induced by interferon-γ, CXCL10\\\/interferon-γ-inducible 10-kd protein, tumor necrosis factor α (TNFα), interferon-γ, vascular endothelial growth factor (VEGF), granulocyte-macrophage colony-stimulating factor, and FasL were measured via a multiplexed cytometric assay. PTX3 and VEGF concentrations were assessed by enzyme-linked immunosorbent assay. PTX3 and CD68 expression were determined by immunohistochemistry and immunofluorescence on temporal artery samples.. RESULTS:. GCA patients with very recent optic nerve ischemia had significantly higher PTX3 and VEGF levels compared to other GCA patients and controls. GCA patients with a disease duration of <6 months had significantly higher PTX3 levels compared to other GCA patients and controls. Immunohistochemistry revealed selective PTX3 expression in the wall of inflamed arteries.. CONCLUSION:. Our findings indicate that local expression of PTX3 is a feature of vascular inflammation in GCA; elevated circulating levels of PTX3 identify patients with very recent optic nerve ischemia or a recent diagnosis. Optic nerve ischemia is also associated with increased circulating VEGF levels.\""
Selective up-regulation of the soluble pattern-recognition receptor pentraxin 3 and of vascular endothelial growth factor in giant cell arteritis: relevance for recent optic nerve ischemia / Baldini, M; Maugeri, N; Ramirez, Ga; Giacomassi, C; Castiglioni, A; Prieto González, S; Corbera Bellalta, M; Comite, Gd; Papa, I; Dell'Antonio, G; Ammirati, E; Cuccovillo, I; Vecchio, V; Mantovani, A; ROVERE QUERINI, Patrizia; Sabbadini, MARIA GRAZIA; Cid, Mc; Manfredi, ANGELO ANDREA M. A.. - In: ARTHRITIS AND RHEUMATISM. - ISSN 1529-0131. - 64:3(2012), pp. 854-865.
Selective up-regulation of the soluble pattern-recognition receptor pentraxin 3 and of vascular endothelial growth factor in giant cell arteritis: relevance for recent optic nerve ischemia.
Castiglioni A;ROVERE QUERINI , PATRIZIA;SABBADINI , MARIA GRAZIA;MANFREDI , ANGELO ANDREA M. A.
2012-01-01
Abstract
"\"OBJECTIVE:. To assess local expression and plasma levels of pentraxin 3 (PTX3) in patients with giant cell arteritis (GCA).. METHODS:. Plasma and serum samples were obtained from 75 patients with GCA (20 of whom had experienced optic nerve ischemia in the previous 3 weeks and 24 of whom had experienced symptom onset in the previous 6 months and had no history of optic nerve ischemia) and 63 controls (35 age-matched healthy subjects, 15 patients with rheumatoid arthritis, and 13 patients with chronic stable angina). In 9 patients in whom GCA was recently diagnosed, circulating levels of interleukin-1β (IL-1β), IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12p70, CCL2\\\/monocyte chemotactic protein 1, CCL3\\\/macrophage inflammatory protein 1α (MIP-1α), CCL4\\\/MIP-1β, CCL11\\\/eotaxin, CXCL9\\\/monokine induced by interferon-γ, CXCL10\\\/interferon-γ-inducible 10-kd protein, tumor necrosis factor α (TNFα), interferon-γ, vascular endothelial growth factor (VEGF), granulocyte-macrophage colony-stimulating factor, and FasL were measured via a multiplexed cytometric assay. PTX3 and VEGF concentrations were assessed by enzyme-linked immunosorbent assay. PTX3 and CD68 expression were determined by immunohistochemistry and immunofluorescence on temporal artery samples.. RESULTS:. GCA patients with very recent optic nerve ischemia had significantly higher PTX3 and VEGF levels compared to other GCA patients and controls. GCA patients with a disease duration of <6 months had significantly higher PTX3 levels compared to other GCA patients and controls. Immunohistochemistry revealed selective PTX3 expression in the wall of inflamed arteries.. CONCLUSION:. Our findings indicate that local expression of PTX3 is a feature of vascular inflammation in GCA; elevated circulating levels of PTX3 identify patients with very recent optic nerve ischemia or a recent diagnosis. Optic nerve ischemia is also associated with increased circulating VEGF levels.\""I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
