Synapsin I is a nerve terminal phosphoprotein which interacts with synaptic vesicles and actin in a phosphorylation-dependent manner. By using fluorescence resonance energy transfer between purified components labeled with fluorescent probes, we now show that the binding of synapsin I to actin is a rapid phenomenon. Binding of synapsin I to actin can also be demonstrated when synaptic vesicles are present in the medium and appears to be modulated by ionic strength and synapsin I phosphorylation.
Rapid binding of synapsin I to F- and G-actin. A study using fluorescence resonance energy transfer / Ceccaldi, P.E., Benfenati, F., Chieregatti, E., Greengard, P., Valtorta, F.. - In: FEBS LETTERS. - ISSN 0014-5793. - 329:3(1993), p. 301-5.
Rapid binding of synapsin I to F- and G-actin. A study using fluorescence resonance energy transfer
VALTORTA, FLAVIAUltimo
1993-01-01
Abstract
Synapsin I is a nerve terminal phosphoprotein which interacts with synaptic vesicles and actin in a phosphorylation-dependent manner. By using fluorescence resonance energy transfer between purified components labeled with fluorescent probes, we now show that the binding of synapsin I to actin is a rapid phenomenon. Binding of synapsin I to actin can also be demonstrated when synaptic vesicles are present in the medium and appears to be modulated by ionic strength and synapsin I phosphorylation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
