Previous studies have shown that the injection of adipose tissue-derived stem cells (ADSCs) into the tunica albuginea (TA) during the active phase of the Peyronie's disease (PD) prevents the development of fibrosis and elastosis in the TA and corpus cavernosum The aim of this study was to investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the TA using a rat model of acute phase of the Peyronie's disease (PD) METHODS: A total of 24 male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (TGFB) and PD treated with SVF (SVF) 1 day after disease induction. Sham rats underwent 2 injections of vehicle into the TA one day apart. TGFB rats underwent TGF- β1 injection and injection of vehicle one day later. SVF rats underwent TGF-β1 injection followed by SVF one day later. One month after treatment all rats underwent measurement of intracorporal pressure (ICP) and mean arterial (MAP) pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were again harvested for histology and Western blot.
Intratunical Injection of Stromal vascular fraction prevents Fibrosis in a Rat Model of Peyronie's Disease / Castiglione, Fabio; Hedlund, Petter; Weyne, Emmanuel; Hakim, Lukman; Montorsi, Francesco; Salonia, Andrea; Bivalacqua, Trinity J; De Ridder, Dirk; Milenkovic, Uros; Ralph, David; Garaffa, Giulio; Muneer, Asif; Joniau, Steven; Albersen, Maarten. - In: BJU INTERNATIONAL. - ISSN 1464-4096. - (2018). [Epub ahead of print] [10.1111/bju.14570]
Intratunical Injection of Stromal vascular fraction prevents Fibrosis in a Rat Model of Peyronie's Disease
Montorsi, Francesco;Salonia, Andrea;
2018-01-01
Abstract
Previous studies have shown that the injection of adipose tissue-derived stem cells (ADSCs) into the tunica albuginea (TA) during the active phase of the Peyronie's disease (PD) prevents the development of fibrosis and elastosis in the TA and corpus cavernosum The aim of this study was to investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the TA using a rat model of acute phase of the Peyronie's disease (PD) METHODS: A total of 24 male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (TGFB) and PD treated with SVF (SVF) 1 day after disease induction. Sham rats underwent 2 injections of vehicle into the TA one day apart. TGFB rats underwent TGF- β1 injection and injection of vehicle one day later. SVF rats underwent TGF-β1 injection followed by SVF one day later. One month after treatment all rats underwent measurement of intracorporal pressure (ICP) and mean arterial (MAP) pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were again harvested for histology and Western blot.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
