Aim: To investigate the transcriptional changes induced by Fingolimod (FTY) in T cells of relapsing remitting multiple sclerosis patients. Patients & methods: Transcriptomic changes after 6 months of FTY therapy were evaluated on T cells from 24 relapsing remitting multiple sclerosis patients through RNA-sequencing, followed by technical validation and pathway analysis. Results: Among differentially expressed genes, CX3CR1 and CCR7 resulted strongly up- and down-regulated, respectively. Two relevant genes were validated with quantitative PCR and we largely confirmed findings from two previous microarray-based studies with similar design. Pathway analysis pointed to an involvement of processes related to immune function and cell migration. Conclusion: Our data support the evidence that FTY induces major transcriptional changes in genes involved in immune response and cell trafficking in T lymphocytes.
Transcriptional effects of fingolimod treatment on peripheral T cells in relapsing remitting multiple sclerosis patients / Sferruzza, Giacomo; Clarelli, Ferdinando; Mascia, Elisabetta; Ferrè, Laura; Ottoboni, Linda; Sorosina, Melissa; Santoro, Silvia; Filippi, Massimo; Provero, Paolo; Esposito, Federica. - In: PHARMACOGENOMICS. - ISSN 1462-2416. - 23:3(2022), pp. 161-171. [10.2217/pgs-2021-0118]
Transcriptional effects of fingolimod treatment on peripheral T cells in relapsing remitting multiple sclerosis patients
Sferruzza, Giacomo;Mascia, Elisabetta;Ferrè, Laura;Filippi, Massimo;
2022-01-01
Abstract
Aim: To investigate the transcriptional changes induced by Fingolimod (FTY) in T cells of relapsing remitting multiple sclerosis patients. Patients & methods: Transcriptomic changes after 6 months of FTY therapy were evaluated on T cells from 24 relapsing remitting multiple sclerosis patients through RNA-sequencing, followed by technical validation and pathway analysis. Results: Among differentially expressed genes, CX3CR1 and CCR7 resulted strongly up- and down-regulated, respectively. Two relevant genes were validated with quantitative PCR and we largely confirmed findings from two previous microarray-based studies with similar design. Pathway analysis pointed to an involvement of processes related to immune function and cell migration. Conclusion: Our data support the evidence that FTY induces major transcriptional changes in genes involved in immune response and cell trafficking in T lymphocytes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
